Why poly a tail

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It is possible that a poly A tail of an appropriate length may function as a platform to recruit mRNA export factors. In this latter scenario, poly A binding protein s should function in recruiting mRNA export factors. The importance of the poly A tail and its binding proteins Pab1p and Nab2p has been already suggested in yeast reviewed in 56, Although the nuclear functions of the yeast poly A binding proteins appear to be related to poly A tail length control and therefore to the above-mentioned exosome-mediated quality control mechanism, rather than to mRNA export per se 58—62 , some studies suggested more direct role of Nab2p in mRNA export 63— The vertebrate homolog of yeast Nab2p, however, has not been identified to our knowledge.

In any case, our finding clearly demonstrates that the poly A tail is directly linked to the mRNA export pathway in vertebrates, and it remains to be investigated whether the poly A tail simply contributes to increasing the RNA length or plays more active role in mRNA export. Google Scholar. Google Preview. Oxford University Press is a department of the University of Oxford.

It furthers the University's objective of excellence in research, scholarship, and education by publishing worldwide. Sign In or Create an Account. Sign In. Advanced Search. Search Menu. Article Navigation. Close mobile search navigation Article Navigation. Volume Article Contents Abstract. Role of poly A tail as an identity element for mRNA nuclear export. Hiroyuki Fuke , Hiroyuki Fuke.

Oxford Academic. Revision received:. Select Format Select format. Permissions Icon Permissions. Abstract Different RNA species are rigorously discriminated and exported by distinct export factors, but this discrimination mechanism remains largely unknown.

Figure 1. Open in new tab Download slide. Figure 2. Figure 3. Figure 4. Figure 5. Figure 6. Figure 7. Google Scholar Crossref. Search ADS. Splicing enhances translation in mammalian cells: an additional function of the exon junction complex. Exon junction complexes mediate the enhancing effect of splicing on mRNA expression. Nuclear export of different classes of RNA is mediated by specific factors. CRM1 is responsible for intracellular transport mediated by the nuclear export signal. The Mex67p-mediated nuclear mRNA export pathway is conserved from yeast to human.

The protein Aly links pre-messenger-RNA splicing to nuclear export in metazoans. SR proteins preferentially associate with mRNAs in the nucleus and facilitate their export to the cytoplasm. The Y14 protein communicates to the cytoplasm the position of exon-exon junctions. The spliceosome deposits multiple proteins 20—24 nucleotides upstream of mRNA exon-exon junctions.

Small nuclear RNA genes: a model system to study fundamental mechanisms of transcription. Rules of engagement: co-transcriptional recruitment of pre-mRNA processing factors. Cotranscriptional coupling of splicing factor recruitment and precursor messenger RNA splicing in mammalian cells. Biochemical analysis of TREX complex recruitment to intronless and intron-containing yeast genes. Characterization and subcellular localization of ribonuclease H activities from Xenopus laevis oocytes.

Google Scholar PubMed. Quality control of messenger ribonucleoprotein particles in the nucleus and at the pore. Poly A -binding proteins: multifunctional scaffolds for the post-transcriptional control of gene expression. Yeast poly A -binding protein Pab1 shuttles between the nucleus and the cytoplasm and functions in mRNA export. A synthetic A tail rescues yeast nuclear accumulation of a ribozyme-terminated transcript. Evidence that poly A binding protein has an evolutionarily conserved function in facilitating mRNA biogenesis and export.

Nuclear export of the yeast mRNA-binding protein Nab2 is linked to a direct interaction with Gfd1 and to Gle1 function. The C-terminal domain of myosin-like protein 1 Mlp1p is a docking site for heterogeneous nuclear ribonucleoproteins that are required for mRNA export.

Issue Section:. Download all slides. Supplementary data. Supplementary Data - zip file. View all The cap and poly A tail function synergistically to regulate mRNA translational efficiency. Abstract The cap structure and the poly A tail are important regulatory determinants in establishing the translational efficiency of a messenger RNA.

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